Abstract: In order to study the anti-fatigue effect of Angelica polysaccharide (APS) on mice, the experimental mice were randomly divided into the swimming control group, the sedentary control group, the high dosage group of polysaccharide (750 mg·kg⁻¹), middle dosage group (500 mg·kg⁻¹) and low dosage group (250 mg·kg⁻¹). The mice in the drug group were treated with intragastric administration for 28 days, and the mice in the control group were treated with the same volume of normal saline. Then, the changes of body weight and the contents of blood urea nitrogen, blood lactic acid, hepatic glycogen, muscle glycogen, glutamic acid and γ-aminobutyric acid in the brain of mice in each group were measured after the swimming test. The results showed that ASP had no significant effect on the body weight of mice (P＞0.05). APS in each dosage group could significantly prolong the exhaustive swimming time of mice (P＜0.05). ASP at the dosage of 250−750 mg·kg⁻¹ reduced the accumulation of blood lactic acid and urea nitrogen in mice after exercise (P＜0.01). The low dosage of APS could significantly promote the increase of glutamic acid level and the decrease of γ-aminobutyric acid level in the hindbrain of mice after the exhaustive exercise for 60 min (P＜0.05), and the ratio of GLU to GABA increased with the increase of APS dosage. APS at the dosage of 750 mg·kg⁻¹ significantly enhanced the storage of hepatic glycogen in mice (P＜0.05). In conclusion, the analysis showed that a certain dose of angelica polysaccharide could effectively reduce the central and peripheral fatigue in mice.