Abstract: In order to explore and screen feasible new methods of the control to maize top rot, the effects of difenoconazole and combined to other bacteriostatic agents were analyzed on the occurrence of maize top rot by seed soaking and seedling spraying. The results showed that seed soaking with difenoconazole（1000 times liquid), difenoconazole（1000 times liquid）added with acid electrolyzed oxidizing water（AEOW ） and AEOW were safe for maize emerging. Their control effects to top rot were 60.49%, 59.79% and 51.75%, respectively. And the control effect of top rot which seedling spraying with difenoconazole（56.25 g a.i.·ha⁻¹), difenoconazole（28.12 g a.i.·ha⁻¹）added with Zhongshengmycin（10.55 g a.i.·ha⁻¹）, and Zhongshengmycin（21.09 g a.i.·ha⁻¹）were 57.42%, 78.22% and 83.04%. Soaking seed and seeding spraying with difenoconazole could significantly inhibit the occurrence of maize top rot, but the control effect of difenoconazole was significantly lower than that of Zhongshengmycin and difenoconazole added with Zhongshengmycin. Therefore, the combination of difenoconazole and Zhongshengmycin could be considered to apply to increase the control effect in field.