Abstract: In order to explore the mechanism of cryptochrome protein of Casuarina equisetifolia in the photoregulatory network, the CRY1 gene sequence of Casuarina equisetifolia was screened and identified from the Casuarina equisetifolia genome database and named CeCRY1, and the full-length cDNA sequence of CeCRY1 gene of Casuarina equisetifolia was cloned by PCR experiment, and the CeCRY1 protein was transiently expressed in tobacco cells by Agrobacterium transformation method for subcellular localization analysis, and bioinformatics analysis of CeCRY1 protein was performed. The results showed that the cDNA of CeCRY1 gene was 2046 bp, the number of amino acids encoded by CeCRY1 gene was 681, the molecular weight of the protein was 76.60 kDa, and the isoelectric point PI was 5.64. The subcellular localization analysis showed that CeCRY1 protein was localized in the nucleus and cytoplasm of tobacco cells. The phylogenetic tree analysis showed that CeCRY1 protein was closely related to CRY1 proteins in some species of Salix family and Morus notabilis of Moraceae family.