Abstract: Bemisia tabaci has become a stubborn pest in the production of Capsicum annuum due to its serious damage and strong resistance to pesticides. In order to clarify the cryptic species of Bemisia tabaci in the greenhouse pepper in Lin’an District of Hangzhou and screen the effective prevention and control agents, the molecular identification and phylogenetic analysis of Bemisia tabaci in this area were carried out. At the same time, five insecticides with different mechanisms of action (50% pymetrozine, 25% methidathion, 5% d-limonene, 17% flupyradifurone and 50 g·L⁻¹ afidopyropen) were selected for the field efficacy trials. The results showed that: the population of Bemisia tabaci in Lin’an District of Hangzhou belonged to the MED cryptic species, which had 100% sequence similarity with the MED cryptic species in Taiwan Province of China and Kochi Prefecture of Japan, and all belonged to the MED Q1 clade. The genetic distance from the MEAM1 cryptic species was significant, with the sequence similarity ranging from 90.2% to 90.6%. The results of field efficacy test showed that 50 g·L⁻¹ afidopyropen and 17% flupyradifurone had the best control effect, and the prevention effect reached more than 80.43% and 75% respectively after 14 days of application. However, the prevention effect of 5% d-limonene was poor, only 31.94% after 14 days of application. The prevention effect of 5% d-limonene was poor, only 31.94% after 14 days. The results provided reference for the prevention and control of Bemisia tabaci in this area.