外源氯化钙对盐胁迫下三角梅生理特性的影响

    Impact of Exogenous Calcium Chloride on the Physiological Characteristics of Bougainvillea spectabilis Under Salt Stress

    • 摘要: 为缓解盐碱地区三角梅盐害问题,丰富滨海盐碱地区植物景观提供参考依据,探究了外源氯化钙(Calcium Chloride,CaCl2)对 NaCl 胁迫下三角梅(Bougainvillea Spectabilis Willd.)的缓解机制。以盆栽三角梅为研究材料,设置1个对照组(CK)和4个处理组,使用150 mmol·L−1NaCl溶液胁迫处理后添加0 mmol·L−1(CK1)、50 mmol·L−1(T1)、100 mmol·L−1(T2)、150 mmol·L−1(T3)浓度的外源氯化钙溶液浇灌胁迫后的三角梅,测定与生长生理相关的一些指标,结合主成分分析和隶属函数分析进行综合评价并筛选出对三角梅盐胁迫缓解效果最佳的氯化钙溶液浓度。结果表明:随着盐胁迫时间延长,三角梅根长、根粗、净光合速率(Pn)和过氧化氢酶(CAT)活性显著降低,脯氨酸(PRO)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛含量(MDA)和相对电导率(REC)显著升高,膜脂过氧化程度加剧。施加CaCl2后,三角梅的根长、根粗、Pn、PRO含量、POD活性、SOD活性和CAT含量均随CaCl2用量的增加呈先升后降趋势,而MDA含量和REC呈先降低后升高的趋势。与CK和CK1处理相比,外源 CaCl2处理后三角梅各指标的变幅均减小,并存在明显剂量效应,即施用适当剂量外源氯化钙可在一定程度上缓解盐胁迫对三角梅的损伤程度,但高水平反而会损伤,其中100 mmol·L−1CaCl2处理对增强三角梅的抗盐性效果最佳。

       

      Abstract: In order to provide a reference for alleviating the salt damage of Bougainvillea spectabilis in the saline-alkali areas and enriching the plant landscape in the coastal saline-alkali areas, the alleviation mechanism of exogenous calcium chloride (CaCl2) on Bougainvillea spectabilis under NaCl stress was explored. By taking the potted Bougainvillea spectabilis as the experimental materials, one control group (CK) and four treatment groups were set up. After subjecting the plants to the stress treatment with 150 mmol·L−1 NaCl solution, the exogenous calcium chloride(CaCl2)solutions at the concentrations of 0 mmol·L−1 (CK1), 50 mmol·L−1 (T1), 100 mmol·L−1 (T2) and 150 mmol·L−1 (T3) were added to irrigate the salt-stressed Bougainvillea spectabilis. Some indexes related to the growth physiology were determined, and the comprehensive evaluation was carried out by combining with the principal component analysis and membership function analysis. Then, the concentration of calcium chloride solution with the best alleviating effect on salt stress of Bougainvillea spectabilis was screened out. The results showed that with the extension of salt stress duration, the root length, root diameter, net photosynthetic rate (Pn), and catalase (CAT) activity of Bougainvillea spectabilis significantly decreased, while the proline content (PRO), superoxide dismutase (SOD) activity, peroxidase (SOD) activity , malondialdehyde (MDA) content, and relative electrical conductivity (REC) significantly increased, indicating an intensified degree of membrane lipid peroxidation. After the application of CaCl2, the root length, root diameter, Pn, PRO content, POD activity, SOD activity and CAT content of Bougainvillea spectabilis initially increased and then decreased with the increase of CaCl2 dosage, while MDA content and REC initially decreased and then increased. Compared with CK and CK1 treatments, the variation amplitude of each index of Bougainvillea spectabilis under the exogenous CaCl2 treatment was reduced, and a clear dose-effect relationship was observed. Specifically, the application of appropriate dose of exogenous calcium chloride could alleviate the damage degree caused by salt stress to Bougainvillea spectabilis to a certain extent, while the high levels of CaCl2 could cause the damage. Among them, the treatment with 100 mmol·L−1 CaCl2 showed the best effect on enhancing the salt tolerance of Bougainvillea spectabilis.

       

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