Abstract: In order to investigate the prevalence and drug resistance of Aeromonas spp. isolated from Micropterus salmoides in Yancheng, 102 diseased samples of M. salmoides were collected from several farms in Yancheng for isolation and culture of Aeromonas. The species of Aeromonas were identified by 16S rRNA gene amplification and sequencing. Six virulence genes, four drug resistance genes and the sensitivity of Aeromonas to eight antimicrobial agents were detected. The results showed that a total of 178 strains of Aeromonas were isolated and identified from the diseased samples of M. salmoides in Yancheng, of which A. veronii and A. hydrophila accounted for 62.36% and 20.79% respectively. The detection rates of the virulence genes ahp and act were 81.46% and 78.09%, respectively; Except ascV and epr, the detection rates of the other four virulence genes were more than 44%. The detection rates of the antibiotics resistance genes flor and qnrs were high, at 37.64% and 31.46%, respectively; The detection rates of ant and tetE were low. The sensitivity rate of Aeromonas isolates to sulfamethoxazole/trimethoprim and enrofloxacin was 60.7% and 59.6%, respectively. The resistance rate to sulfamonomethoxine sodium was the highest, which was 82%.