Abstract: Abstracts: To explore the feasibility of using bamboo shoot shells as a substitute for sawdust in Ganoderma lucidum cultivation, develop a bamboo shoot shell-based cultivation substrate, and reduce environmental pollution caused by bamboo shoot shells, this study investigates the effects of different substitution ratios of bamboo shoot shells for sawdust in G. lucidum cultivation. The experiment includes four substitution ratios: 0%, 25%, 50%, and 75%. The results showed that bamboo shoot shell substitution for sawdust reduced the growth rate of G. lucidum, and the inhibitory effect became more pronounced with increasing substitution ratios. Replacing 25% of sawdust with bamboo shoot shells had no significant effect on the fruiting time of the two G. lucidum, but the biological efficiency, total triterpenes, and total polysaccharide content of G.lucidum（Dahongzhi）and G. lucidum Nanzhi No. 1 increased significantly by 13.14% and 7.23%, 13.50% and 68.55%, and 16.49% and 35.48%, respectively. Total amino acid content was highest at the 50% substitution rate, followed by 25%. The C/N ratio of the cultivation substrate was positively correlated with biomass and mycelial growth rate, but negatively correlated with total polysaccharides and amino acid content. In conclusion, substituting 25% of sawdust with bamboo shoot shells in G. lucidum cultivation ensures yield while improving quality.