Abstract: Rice is the most important food crop in China, and fertilization is an important agronomic measure to increase rice yield and maintain soil fertility. In order to explore the effects of different fertilizer applications on the growth traits and nutrient absorption of rice, a fixed-location experiment with three different fertilizer application methods, namely pure chemical fertilizer（CF）with equal input of nitrogen, phosphorus and potassium, 50% organic fertilizer replacing chemical fertilizer（COF）, and biochar-based fertilizer（CBF）, was carried out in Huzhou City, Zhejiang Province, and compared with the treatment without fertilization（CK）. he results indicated that, Fertilization significantly increased rice plant height, panicle length, effective panicle number, as well as grain and straw yields（P＜0.05）, with grain and straw yields increasing by 81.3%-93.4% and 85.4%-96.6%, respectively. However, no significant differences were observed among CF, COF and CBF treatments（P＞0.05）.Fertilization significantly enhanced nitrogen（N）, phosphorus（P）and potassium（K）uptake in rice plants（P＜0.05）. Compared with the control, N uptake in grains and straw increased by 100.7%-123.1% and 102.3%-118.6%, P uptake increased by 192.5%-112.8% and 97.5%-110.2%, and K uptake increased by 84.4%-104.8% and 87.2%-101.3%, respectively. Nevertheless, no significant differences were found among CF, COF and CBF treatments（P＞0.05）.Compared with CF, COF significantly improved N and P uptake efficiency by 19.5% and 18.1%（P＜0.05）, while CBF significantly enhanced N and K uptake efficiency by 17.4% and 16.9%（P＜0.05）. Additionally, the agronomic efficiency of COF and CBF increased by 14.9% and 13.5%, respectively, compared with CF. In conclusion, the application of organic fertilizer substitution for chemical fertilizers and biochar-based fertilizers can be promoted in future rice production.