一个水稻重组自交系群体的全基因组选择模型分析

    Genome-wide selection analysis of a rice recombinant inbred line populations

    • 摘要: 为了更好的在育种中利用一个由蜀恢527和绵恢436杂交构建的重组自交系(RIL)群体,构建了该群体的全基因组选择(GS)模型,并评估出最优模型用于将来筛选强优恢复系,以提高育种效率。在检测了该群体数量农艺性状及基因型后构建了3个GS模型,通过评估GS模型在RIL群体的各类性状上的表现,探究了3个预测模型在该群体中应用的准确性和预测能力。结果表明:水稻RIL群体的抽穗期、有效分蘖数、株高、粒长、粒宽、穗长、每穗实粒数、单株产量等8个性状均为多基因控制的数量性状,可用来做GS分析。利用高通量测序获得群体的基因型,经筛选过滤后,获得了181份样本的454 129个SNPs并构建了3个预测模型。从模型的准确度和预测能力两个方面评估,GBLUP模型总体表现优秀可用于后续使用。

       

      Abstract: To better utilize a recombinant inbred line(RIL)population derived from the cross between Shuhui527 and Mianhui436 in breeding programs, we established genomic selection(GS)models for this population and identified the optimal model for future screening of superior restorer lines to enhance breeding efficiency. Following comprehensive phenotyping of agronomic traits and genotyping of the population, three GS models were developed. The predictive accuracy and performance of these models across multiple traits in the RIL population were systematically evaluated to explore their applicability. The results showed that: eight quantitative traits in rice - heading date, productive tillers per plant, plant height, grain length, grain width, panicle length, grain number per panicle, and yield per plant - exhibited polygenic inheritance patterns, making them suitable for GS analysis. High-throughput sequencing generated genotype data yielding 454,129 filtered SNPs from 181 accessions, which were used for model construction. Comprehensive evaluation based on model accuracy and predictive capacity revealed that the GBLUP model exhibited superior overall performance, demonstrating its potential for practical implementation in subsequent breeding applications.

       

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