Abstract: This study aimed to identify the genetic diversity and evolutionary characteristics of Citrus tatter leaf virus（CTLV）, the whole genomes of 41 CTLV isolates from 7 different citrus species were analyzed by genetic diversity and evolution. The result suggested that the base composition of 41 CTLV isolates from 7 different citrus species shows AT bias, the ratio of base conversion to transposition is 2.875～5.112, the Pi value of nucleotide diversity is 0.030～0.177, and the Hd value of haplotype diversity is 0.900～1.000. The neutral test values are mostly negative and not significant, indicating that the populations has not experienced. The ratio of non-synonymous mutations to synonymous mutations is 0.059～0.754, indicating negative selection. The F_ST value in Molecular Variation Analysis（AMOVA）is 0.193, indicating high genetic differentiation between populations, with the variation mainly originating from within populations. Phylogenetic analysis shows that there is no significant host specificity in the clustering of CTLV isolates. This study showed that 41 CTLV isolates have high nucleotide and haplotype diversity, with gene mutations and negative selection being key drivers of their genetic evolution, while host differences have little impact on CTLV genetic evolution.