Abstract: In order to optimize the tissue culture and rapid propagation technology system of Polygonatum cyrtonema Hua, 70% mature berries, mature seeds, and the underground tubers of three-year-old seedlings of Polygonatum cyrtonema Hua were used as the experimental materials to screen the best explants. The further experiments were conducted to select the optimal induction medium, bud propagation subculture medium, and rooting medium for the best explants. At the same time, the application effects of glass bottles and high-pressure resistant transparent bags were compared in the intensive production of Polygonatum cyrtonema Hua. The results showed that the optimal explant of Polygonatum cyrtonema Hua was mature seeds, which had the highest induction rate in the medium of MS+6-BA2 mg·L⁻¹+GA₃0.30 mg·L⁻¹+NAA0.05 mg·L⁻¹+sucrose 30 g·L⁻¹. The proliferation effect was better in the medium of MS+6-BA 1.6 mg·L⁻¹+Qingjunyi 0.07 g·L⁻¹+sucrose 30 g·L⁻¹, with the proliferation coefficient of over 4. The rooting effect was better in the rooting medium of MS+NAA0.3 mg·L⁻¹+IBA0.6 mg·L⁻¹+sucrose 30 g·L⁻¹, with the rooting rate of 95%. At the same time, the experiments had shown that the bag-type tissue culture could be used instead of the bottle-type tissue culture for the entire growth cycle of intensive production of Polygonatum cyrtonema Hua seedlings.