Abstract: In an artificial breeding setting, striped bamboo sharks exhibited abnormal behavior, agitation, and mortality. To identify the causative pathogen and screen for effective therapeutic agents, a dominant bacterial strain designated CV-P1 was isolated from the spleen tissue of affected sharks. Through morphological observation, physiological and biochemical characterization, 16S rRNA gene sequencing, phylogenetic tree construction, and challenge infection tests, strain CV-P1 was identified as Vibrio harveyi, with a median lethal dose（LD₅₀）of 1.0×10⁵ CFU·mL⁻¹. The drug susceptibility test indicated that the bacterium was susceptible to 12 antibiotics, including enrofloxacin, neomycin, and thiamphenicol, moderately susceptible to colistin, and resistant to 9 antibiotics, such as florfenicol and tetracycline.Furthermore, the minimum inhibitory concentration（MIC）of each antimicrobial agent against strain CV-P1 was determined using the microdilution method. The results indicated that flumequine, sulfisoxazole, ceftiofur, ceftazidime, and ofloxacin all had an MIC of 0.25 μg·mL⁻¹; while the MIC values for enrofloxacin, neomycin, thiamphenicol, doxycycline, sulfamonomethoxine, amoxicillin/clavulanic acid（augmentin）, and gentamicin were 0.03, 2, 1, 0.125, 2, 2/1, and 0.5 μg·mL⁻¹, respectively. This study represents the first report of V. harveyi isolated from diseased striped bamboo sharks, and the results may provide valuable insights for the prevention and treatment of bacterial diseases in this species.