Abstract: Under the background of the national comprehensive promotion of rural revitalization strategy, as a key medium for connecting the research and development of agricultural science and technology with the production practice, the agricultural journals are undergoing a profound transformation from traditional content production to digitization, platformization, servitization and precision. By taking Southeast Horticulture as the research object, the innovative pathways for developing the distinctive journal column were deeply explored from the aspects of column positioning, editorial capacity building, digital dissemination, and collaborative mechanisms with science and technology correspondent, thus to realize the transformation from “theoretical suspension” to “practical anchoring”. The successful practices of Southeast Horticulture provided replicable experience for the similar journals in their pursuit of distinctive column development.