Abstract: To optimize tissue culture rapid propagation technology for soapwort, young stems, leaves, and leaf axils were used as experimental materials. The MS medium supplemented with 6-BA（2.0 mg·L⁻¹）+ 2,4-D（0.5 mg·L⁻¹）+ 10% coconut milk was employed to identify the optimal explant sites and sterilization duration. Further, an L₉(3⁴) orthogonal test was conducted using MS, 1/2MS, and WPM as base media. By varying concentrations of 6-BA and NAA and adjusting sterile water-holding times, the optimal medium for axillary explant growth and the best formulation for inducing green callus were identified. Growth performance of soapwort tissue-cultured seedlings under different media and hormone combinations was also compared. Results indicate: The optimal explant site for soapwort is the leaf axil, with an optimal sterilization time of 8 min yielding the highest survival rate of 58.58%. The optimal growth medium combination for leaf axils was 1/2 MS + 6-BA（0.5 mg·L⁻¹）+ NAA（0.05 mg·L⁻¹）+ sterile water control for 3 days, yielding the highest callus formation rate of 20%, surpassing other treatments. The optimal medium combination for inducing green callus from leaf axils was WPM + 6-BA（0.1 mg·L⁻¹）+ NAA（0.2 mg·L⁻¹）+ sterile water control for 3 days, yielding a 60% callus formation rate. These findings provide a reference for establishing an efficient regeneration system and tissue culture techniques for soapwort, while also offering high-quality seedlings and technical guidance for its industrialized propagation.