Abstract: In order to screen the potato germplasm resources with high vitamin C content, the vitamin C content of 52 potato germplasm resources collected from 10 breeding institutions in China was determined, and the variation analysis and cluster analysis were carried out. The results showed that the vitamin C content of the 52 potato germplasm resources ranged from 19.5 to 56.1 mg·hg⁻¹, with an average of 36.5 mg·hg⁻¹, and the coefficient of variation was 19.8 %. The results of variance analysis showed that the difference of vitamin C content among different germplasm resources was extremely significant, indicating that there were abundant genetic variations among the tested materials. The 52 tested materials were divided into five categories by cluster analysis. Among them, the high vitamin C category contained two germplasm resources, namely Tuyan No.2 and Zhongshu 26, with the vitamin contents of 56.1 and 53.2 mg·hg⁻¹, respectively. In addition, there were 7 germplasm resources in the higher category, with a content ranging from 43.2 mg·hg⁻¹ to 49.7 mg·hg⁻¹. The high vitamin C germplasm resources screened in this study could provide material foundations for potato nutritional quality breeding and for elucidating the mechanisms of vitamin C accumulation.