利用显性核不育水稻构建开放式轮回选择育种方法

    Developing an Open-Ended Recurrent Selection Breeding Approach with Dominant Genic Male Sterile Rice

    • 摘要: 显性核不育水稻是构建混交育种群体开展轮回选择的理想载体。选择4个典型的粳稻不育系分别与6份籼粳交恢复系测配,以F1的结实率不低于85%的标准筛选出5份高亲和力的恢复系。利用高亲和力恢复系作为轮回亲本与显性核不育系回交获得BC3F1 群体,该群体每一世代会分离出等比例的可育株和不育株。可育株经过系谱选择和耐逆抗性筛查,经测配后可以作为新种质加入到供体父本群中,这是一条经典的传统轮回选择路径。而不育株则可以作为桥梁,每一轮杂交都可以引入新种质,这样可以创建一个开放式的轮回选择群体。并将此方法运用到水稻高亲和力恢复系的选育上,从该群体中获得了108份F3~F7代候选株系,并探讨了开放式轮回选择与传统封闭式轮回选择的优劣。

       

      Abstract: Dominant Genic Male Sterile rice(DGMS)provides an ideal vehicle for establishing multiple inter-cross population for recurrent selection. In this study, four typical japonica rice male sterile lines were test-crossed with six indica-japonica restorer lines, and five high-compatibility restorer lines were selected based on a seed setting rate above 85% in the F1 hybrids. Using these high-compatibility restorer lines as recurrent parents, they were backcrossed with a dominant genic male sterile line to obtain a BC3F1 population, which segregated into fertile and sterile plants at an equal ratio in each generation. The fertile plants, after pedigree selection and screening for stress tolerance, could be incorporated as new germplasm into the donor male parent pool following test-crossing, representing a classical pathway of traditional recurrent selection. In contrast, the sterile plants could serve as a bridge to introduce new germplasm in each crossing round, thereby establishing an open-ended recurrent selection population. This method was applied to the breeding of high-compatibility restorer lines in rice, and 108 candidate lines from the F3 to F7 generations were obtained from this population. The advantages and disadvantages of open-ended recurrent selection versus traditional closed recurrent selection were also discussed.

       

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