Abstract: In order to explore the effect of combining the soil fumigant dazomet with biological agents on alleviating the continuous cropping obstacles in pepper, Zaoxiu pepper was used as the test material, and four treatments were set up: single application of dazomet (CK), dazomet + Lvheng 168 (MLH), dazomet + Taifeng 1 (MTF) and dazomet + Yibingqing (MYBQ). Each treatment was repeated for three times. Then, the survival rate, growth index, yield and fruit quality of pepper were measured. The results showed that the survival rates of pepper treated with MYBQ and MTF were 68.97% and 66.67%, respectively, which were significantly higher than those of CK (46.67%) and MLH (41.07%) treatments. Compared with CK, the MLH treatment significantly increased the plant height and stem diameter of pepper, while the MTF and MYBQ treatments significantly increased the chlorophyll content of pepper leaves. The yield of pepper treated with MTF and MYBQ reached 35 541.00 kg·hm⁻² and 33 486.75 kg·hm⁻², respectively, which were 47.0% and 38.5% higher than that of CK. The soluble protein and total phenolic content of pepper fruit treated with MTF were the highest, indicating the most pronounced improvement in fruit quality. In conclusion, the combination of dazomet and Taifeng 1 demonstrated the best effect on increasing yield and improving quality, while the combination of dazomet and Yibingqing achieved the highest survival rate along with a significant yield increase. Both of them could be used as effective combination techniques for alleviating the continuous cropping obstacles in pepper.