基于Probit模型分析茶皂素对非洲大蜗牛的毒力效应

    Probit Model-Based Toxicity Evaluation of Tea Saponin against Achatina fulica

    • 摘要: 为明确茶皂素对非洲大蜗牛的防控效果,采用室内喷雾法测定0.25%、0.5%、1.0%、1.5%、2.0%和2.5%浓度的茶皂素对非洲大蜗牛的灭杀效果,以蒸馏水为空白对照、6%四聚乙醛颗粒剂为阳性对照。结果表明:茶皂素对非洲大蜗牛的杀螺效果随处理浓度升高和处理时间延长而增强,茶皂素浓度由0.25%升至2.5%,72 h校正死亡率由26.7%升至93.1%,2.5%茶皂素处理与6%四聚乙醛(阳性对照组)的防效相当,二者间无显著差异;基于Probit模型的毒力评估显示,LC50随处理时间显著下降,24、48和72 h分别为2.69%、1.16%和0.65%;72 h剂量-效应拟合曲线呈标准S型,LC50位于曲线快速上升区段,拟合效果良好。综上,茶皂素具备开发为防治非洲大蜗牛植物源杀螺剂的潜力。

       

      Abstract: To clarify the control efficacy of tea saponin against Achatina fulica, the molluscicidal effects of tea saponin at concentrations of 0.25%, 0.5%, 1.0%, 1.5%, 2.0%, and 2.5% were evaluated using a laboratory spray method, with distilled water as the blank control and 6% metaldehyde granules as the positive control. The results showed that the molluscicidal effect of tea saponin against A. fulica increased with increasing concentration and prolonged treatment time. At 72 h, as the concentration increased from 0.25% to 2.5%, the corrected mortality rose from 26.7% to 93.1%, which was comparable to that of the positive control(6% metaldehyde). Probit-based toxicity evaluation revealed that the LC50 decreased significantly with treatment time, with values of 2.69%, 1.16%, and 0.65% at 24, 48, and 72 h, respectively. The 72 h dose-response curve fitted by the Probit model exhibited a standard sigmoidal shape, with the LC50 located in the rapidly ascending segment and showing good goodness-of-fit. Collectively, these findings indicate that tea saponin has potential for development as a plant-derived molluscicide for the control of Achatina fulica.

       

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