水稻籼粳属性分类方法及应用研究进展

    Research progress on identification methods and application of indica-japonica characteristics in Rice

    • 摘要: 籼粳杂交育种是提高水稻产量的有效途径之一,准确鉴定亲本材料的籼粳属性,是高效利用稻种资源、挖掘亚种间杂种优势、培育强优势组合的核心基础。近70年来,籼粳属性鉴定方法经历了从宏观形态观测到微观基因组解析的演进历程。文章综述了几种不同水稻籼粳属性分类方法的技术原理、优缺点及应用情况。其中,程式指数法是最典型的分类方法,其简单、直观,常用于田间调查;分子标记法中,SSR、InDel是最常用的标记,其结果更准确、可靠,基于全基因组重测序,SNP标记的高通量筛选,实现了籼粳属性的精准定量。

       

      Abstract: Indica-japonica hybrid breeding is one of the effective approaches for increasing rice yield, accurately identifying the indica/japonica attributes of parental materials serves as the core foundation for efficient utilization of rice germplasm resources, exploiting inter-subspecific heterosis, and cultivating strong heterotic combinations. Over the past nearly 70 years, methods for identifying indica/japonica attributes have evolved from macroscopic morphological observation to microscopic genomic analysis. This paper describes the technical principles, advantages, limitations, and application status of several classification methods for determining indica/japonica attributes in rice. Among them, the Cheng's index method is the most typical classification approach, which is simple and intuitive, and is commonly used in field investigations, in molecular marker methods, SSR and InDel are the most frequently used markers, yielding more accurate and reliable results, based on whole-genome resequencing, high-throughput screening of SNP markers has enabled precise quantification of indica/japonica attributes.

       

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