Abstract:
To explore novel microbial resources applicable to feed improvement and the production of functional enzyme preparations, this study used accumulated soil samples from camellia oil mills and camellia oil meal as isolation materials. Twenty-three strains capable of decomposing phospholipids and producing hydrolytic clear zones were screened, from which strain FL-11, a high-yield producer of phospholipase C(PLC), was selected. The strain was identified through an analysis of colony morphology, physiological and biochemical characteristics, and 16S rDNA sequence homology. The results showed that strain FL-11 shared a 99.5% homology with
Pseudomonas baetica and was thus named
Pseudomonas baetica FL-11. The components of the fermentation medium for enzyme production were optimized using single-factor experiments combined with response surface methodology. The optimal medium formulation was determined as follows: glucose 22.8 g·L
−1, corn steep liquor 11.2 g·L
−1, and 20% egg yolk emulsion at an addition level of 1%. Under these culture conditions, the PLC activity of the fermentation broth of this strain reached 17.45 U·mL
−1.