81份马铃薯种质资源表型性状遗传多样性分析及评价

    Analysis and evaluation of phenotypic trait genetic diversity of 81 potato germplasm resources

    • 摘要: 对国内外马铃薯种质资源进行农艺性状鉴定及评价,以期筛选适宜泉州地区春种高温高湿环境的优异种质。在本地区进行春种田间试验,对81份种质资源的8个质量性状和4个数量性状进行遗传多样性分析。结果表明:供试马铃薯种质的8个质量性状变异系数范围为1.20%~82.70%,其中薯皮光滑度的变异系数最大;Shannon-weaver多样性指数(H')为0.577~1.634,H'高于1的质量性状为花冠颜色、熟性、薯皮色、薯肉色和结实性。供试马铃薯种质的4个数量性状变异系数为12.03%~39.99%,单株结薯数、单株产量和小区产量的变异程度较大,种质间性状差异明显;商品薯率的变异最低,性状遗传较为稳定。相关性分析结果显示,小区产量与单株产量、商品薯率呈极显著正相关;商品薯率与小区产量呈极显著正相关;单株结薯数与商品薯率呈极显著负相关。经主成分分析提取5个主成分,累计方差贡献率为71.662% ,前3个主成分涵盖小区产量、单株产量、商品薯率、结实性、花冠颜色与芽眼性状。经聚类分析,供试种质划分为3个大类群,其中第Ⅲ大类群又分为4个亚群。第Ⅰ大类群可用于丰产性与薯肉色改良;第Ⅱ大类群可用作早熟品种选育的核心亲本材料;第Ⅲ大类群中,第Ⅰ亚类群可提供特色彩色、丰产选育的亲本材料,第Ⅱ亚类群适用于结薯数搭配高商品薯率的定向选育,第Ⅲ亚类群可作为核心育种骨干亲本,第Ⅳ亚类群可用于适配当地熟性、合理结薯数与高商品薯率的鲜食品种选育。这些种质资源既为泉州地区马铃薯育种拓宽了亲本遗传背景,也为后续相关分子机制研究积累了系统的表型基础数据。

       

      Abstract: Conduct identification and evaluation of agronomic traits for potato germplasm resources both domestically and internationally. In order to screen excellent germplasm suitable for the high-temperature and high-humidity environment of spring planting in Quanzhou area. Conduct spring field trials in this region, Genetic diversity analysis was conducted on 8 quality traits and 4 quantitative traits of 81 germplasm resources. The results showed that the coefficient of variation for the eight quality traits of the tested potato germplasm ranged from 1.20% to 82.70%. Among them, the coefficient of variation for the smoothness of sweet potato skin was the largest. The Shannon-Weaver diversity index(H')ranges from 0. 577 to 1.634. The qualitative traits with H' values higher than 1 were corolla color, maturity, skin color, flesh color, and seed setting ability. The coefficient of variation for the four quantitative traits of the tested potato germplasm ranged from 12.03% to 39.99%. The degree of variation in tuber number per plant, yield per plant, and yield per plot were relatively high. There were significant differences in traits among germplasms. The variation in the rate of marketable potatoes was the lowest. The inheritance of traits was relatively stable. The correlation analysis results showed that the yield per unit area was significantly positively correlated with the yield per plant and the rate of marketable potatoes. The marketable potato rate was highly significantly positively correlated with the yield per plot. The number of tubers per plant was significantly negatively correlated with the rate of marketable tubers. Extract 5 principal components through principal component analysis. The first three principal components encompassed yield per plot, yield per plant, marketable tuber rate, seed setting ability, corolla color, and bud eye traits. Through cluster analysis, The tested germplasm was divided into three major groups. The third major group was further divided into four subgroups. The first major group could be utilized for improving fertility and flesh color of sweet potatoes. The second major group could be used as core parental materials for the breeding of early-maturing varieties. In the third major group, The first subgroup could provide parental materials with distinctive color and high yield for breeding. Sub-group II was suitable for targeted breeding with a combination of tuber number and high marketable tuber rate. The third subgroup could serve as the core breeding backbone parent. The fourth subgroup could be utilized for the breeding of fresh food varieties that were adapted to local maturity, had a reasonable number of tubers, and exhibit a high rate of marketable tubers. These germplasm resources had broadened the parental genetic background for potato breeding in the Quanzhou region. They also accumulated systematic phenotypic basic data for subsequent research on related molecular mechanisms.

       

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