Abstract: According to the measured data of the chlorophyll content and using the analysis techniques of original spectrum and derivative spectra, the spectral characteristic parameters (DV₆₄₀, R₇₁₆) for estimating chlorophyll content in the fresh leaf of tea plant “Tieguanyin” were obtained and the spectral parameter model for estimating chlorophyll content was established. The results showed that the estimation accuracy of the model established by DV₆₄₀ parameters of the fifth and sixth leaves could reach a higher value of 93%.