Abstract: With the specific primes for internal transcribed spacer of Bursaphelenchus xylophilus rDNA, nematode genomic DNA carried in the imported pine wood platelets from Quanzhou Port were specifically amplified with PCR method in order to set up a quick and accurate detection way for nematodes. The results applied this detection way in Quanzhou Port showed that no Bursaphelenchus xylophilus was found, which was consistent with the results detected by traditional morphology method.