Abstract: A cultivation experiment of straw mushroom cv. “9715” was carried out using fresh edible fungi residues of needle mushroom, Eryngii mushroom and seafood mushroom, as well as corncob as cultivation medium in order to explore the potential resource of substitute medium material for culturing straw mushroom. The results showed that the yield and biotransformation rate of straw mushroom using needle mushroom residue were higher than that using other mushrooms residues. In conclusion, needle mushroom residue could be used as substitute material for straw mushroom cultivation.