Abstract: With seafood mushroom waste medium material which from factory production as the main raw material, taking bag planting mode and designing a number of different formulations and different sterilization conditions, the effects of which on the yield of Agaricus bisporus were studied. The results showed that using the waste and sterilized medium material of seafood mushroom to plant Agaricus bisporus in bags could get better returns, and a suitable cultivation formula and sterilization condition were as follows: seafood mushroom waste 50 kg, cow dung 10 kg, calcium superphosphate 0.50 kg, light calcium carbonate 0.25 kg, lime 0.06 kg, water content 64%, disinfection temperature was121℃ for 2 h.