一种利用碱裂解法快速筛选重组子的方法

吕新; 陈丽华; 李玥仁

doi:10.13651/j.cnki.fjnykj.2016.05.005

吕新, 陈丽华, 李玥仁. 一种利用碱裂解法快速筛选重组子的方法[J]. 福建农业科技, 2016, 47(5): 13-15. DOI: 10.13651/j.cnki.fjnykj.2016.05.005

引用本文:

吕新, 陈丽华, 李玥仁. 一种利用碱裂解法快速筛选重组子的方法[J]. 福建农业科技, 2016, 47(5): 13-15. DOI: 10.13651/j.cnki.fjnykj.2016.05.005

LV Xin, CHEN Li-hua, LI Yue-ren. A rapid screening method of recombinant methods by using alkaline lysis[J]. Fujian Agricultural Science and Technology, 2016, 47(5): 13-15. DOI: 10.13651/j.cnki.fjnykj.2016.05.005

Citation:

LV Xin, CHEN Li-hua, LI Yue-ren. A rapid screening method of recombinant methods by using alkaline lysis[J]. Fujian Agricultural Science and Technology, 2016, 47(5): 13-15. DOI: 10.13651/j.cnki.fjnykj.2016.05.005

一种利用碱裂解法快速筛选重组子的方法

A rapid screening method of recombinant methods by using alkaline lysis

摘要

摘要: 根据碱裂解法提取质粒DNA的原理，利用碱裂解法快速筛选到重组子，该方法快迅、准确，可应用于基因克隆中的重组菌落快迅检测。

Abstract: According to the principle of alkaline lysis method for extracting plasmid DNA, the recombinants were rapidly screened by using the alkaline lysis method, which is quick and accurate and can be applied in rapid detection of recombinant bacterial colonies in gene cloning.

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