Abstract: In this paper, the growth conditions of vegetables Chinese wolfberry "Yeyong 2" taking the waste material of Pleurotus eryngii and Pleurotus geesteranus as the substrates were studied by soilless cultivation method. The results showed that "Yeyong2" had higher cutting survival rate which taking the waste material of Pleurotus eryngii as the substrates and the treatment 3 was highest, reaching 92.7%. And the fresh weight of Chinese wolfberry treated by waste material of Pleurotus geesteranus was significantly higher than that of Pleurotus eryngii. "Yeyong 2" had higher protein, crude ash, calcium, iron and other nutrients, and there is great difference for moisture content and Vc content among different treatments, but little difference for crude protein, crude ash, Ca and Fe.