Abstract: In this paper, fresh-keeping effects of different ratio of clove, astragalosides and justicia extracts on Chinese black olive were studied through in vitro bacteriostasis experiment and storage in vivo. The results showed that the best bacteriostatic efficacy were obtained when ratios of clove, astragalosides and justicia extracts were 3:1:3, 3:1:2 and 3:3:1, respectively. These extracts could delay rot of Chinese black olive, lower weight loss ratio and prevent reduction of Vc content.