Abstract: Effects of different N fertilizer-applying method on the growth and yield of machine-harvested ratooning rice during regeneration season were studied. The results showed that using "Jiayou 99" as machine-harvested ratooning rice, applying urea 5 kg/667 m² (75 kg/hm²) as bud fertilizer after 20 days of full heading in the first season, applying urea 20 kg/667 m²(300 kg/hm²) as seedling-promoting fertilizer after 2 days of harvesting, and applying urea 5 kg/667 m²(75 kg/hm²) as the fertilizer for full growth of seedlings after 10 days of harvesting the first season crop, this method could obtain higher yield and output of ratooning rice.