Abstract: The Juncao, corncob, bagasse and cotton seed hull were used to partly substitute sawdust in the cultivation of black fungus, and six formulas were designed to study their effects on the growth and quality of black fungus. The results showed that formula 4 (32% sawdust+21% corncob+21% bagasse+12% cotton seed hull) and formula 5 (32% sawdust+21% Juncao+21% bagasse+12% cotton seed hull) were confirmed as the best culture formulas, which had better effects on the mycelium growth and the yield of black fungus.