Abstract: A single factor test and orthogonal design were used to study the optimization conditions of the nitrogen source, inorganic salt, sawdust extract of Antrodia camphor, the addition of VB and other factors in the liquid fermentation culture of Taiwan Antrodia camphorata. Single factor experiments showed that the culture medium with soybean cake powder is more beneficial to the fermentation of Antrodia camphorata than with potato starch. Under the same feeding amount, the growth effect of Antrodia camphorata added with K⁺ salt was better than that of Na⁺ salt. The orthogonal experiment showed that the optimum culture conditions for liquid fermentation of Antrodia camphorata were glucose 30 g·L^-1, soybean cake powder 30 g·L^-1, potassium dihydrogen phosphate 1.5 g·L^-1, camphor wood scraps extract 9 ml·L^-1.