王翠, 郭仲杰, 尤洁, 宋飞飞, 蔡志欣. 双孢蘑菇产、质量性状相关分子标记的初步研究[J]. 福建农业科技, 2018, 49(7): 1-5. DOI: 10.13651/j.cnki.fjnykj.2018.07.001
    引用本文: 王翠, 郭仲杰, 尤洁, 宋飞飞, 蔡志欣. 双孢蘑菇产、质量性状相关分子标记的初步研究[J]. 福建农业科技, 2018, 49(7): 1-5. DOI: 10.13651/j.cnki.fjnykj.2018.07.001
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    WANG Cui, GUO Zhong-jie, YOU Jie, SONG Fei-fei, CAI Zhi-xin. A Preliminary Study on Molecular Markers Related to Yield and Quality Traits of Agaricus bisporus[J]. Fujian Agricultural Science and Technology, 2018, 49(7): 1-5. DOI: 10.13651/j.cnki.fjnykj.2018.07.001
    Citation: WANG Cui, GUO Zhong-jie, YOU Jie, SONG Fei-fei, CAI Zhi-xin. A Preliminary Study on Molecular Markers Related to Yield and Quality Traits of Agaricus bisporus[J]. Fujian Agricultural Science and Technology, 2018, 49(7): 1-5. DOI: 10.13651/j.cnki.fjnykj.2018.07.001
    shu

    双孢蘑菇产、质量性状相关分子标记的初步研究

    A Preliminary Study on Molecular Markers Related to Yield and Quality Traits of Agaricus bisporus

      摘要
    • 摘要: 对206个收集自世界各地的双孢蘑菇栽培菌株的总DNA进行大规模的SRAP、ISSR和RAPD分析，共获得15条SRAP、3条ISSR和2条RAPD特异性标记条带。利用这20个标记条带对206个菌株中的具有代表性的3种不同农艺性状的131个菌株进行统计和分析，获得标记和相关性状的相关性。结果表明：试验共筛选到5个与双孢蘑菇质量性状明显相关的标记SRAP1-51000、SRAP2-3200、SRAP5-9650、SRAP5-10400、ISSR8092100；3个与双孢蘑菇产量性状明显相关的标记SRAP2-3400、SRAP2-41200、SRAP2-31800。研究获得的分子标记经下一步的验证，有望作为双孢蘑菇的杂交育种过程中高产、优质菌株筛选预测的特异性分子标记。

       

      Abstract: In this paper, a large scale of SRAP, ISSR and RAPD analyses on DNAs from 206 cultivated strains of Agaricus bisporus collected from around the word were carried out. 15 specific SRAP bands, 3 specific ISSR bands and 2 specific RAPD bands were obtained. 131 strains with three different agronomic traits among 206 strains were analyzed using the above 20 specific bands to study relation between markers and traits. The results showed that there were five markers closely related to quality traits, including SRAP1-51000, SRAP2-3200, SRAP5-9650, SRAP5-10400 and ISSR8092100 and three markers closely related to yield traits, including SRAP2-3400, SRAP2-41200 and SRAP2-31800. All the above related markers should be further validated and expected to become the specific molecular markers for screening and predicting of high yield and high quality strains in cross breeding of Agaricus bisporus.

       

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