周淑芬, 刘华清. 水稻OsTZF3基因敲除和过量表达载体的构建与遗传转化[J]. 福建农业科技, 2019, 50(3): 1-5. DOI: 10.13651/j.cnki.fjnykj.2019.03.001
    引用本文: 周淑芬, 刘华清. 水稻OsTZF3基因敲除和过量表达载体的构建与遗传转化[J]. 福建农业科技, 2019, 50(3): 1-5. DOI: 10.13651/j.cnki.fjnykj.2019.03.001
    shu
    ZHOU Shu-fen, LIU Hua-qing. Construction and Genetic Transformation of OsTZF3 Gene Knockout and Overexpression Vectors in Rice[J]. Fujian Agricultural Science and Technology, 2019, 50(3): 1-5. DOI: 10.13651/j.cnki.fjnykj.2019.03.001
    Citation: ZHOU Shu-fen, LIU Hua-qing. Construction and Genetic Transformation of OsTZF3 Gene Knockout and Overexpression Vectors in Rice[J]. Fujian Agricultural Science and Technology, 2019, 50(3): 1-5. DOI: 10.13651/j.cnki.fjnykj.2019.03.001
    shu

    水稻OsTZF3基因敲除和过量表达载体的构建与遗传转化

    Construction and Genetic Transformation of OsTZF3 Gene Knockout and Overexpression Vectors in Rice

      摘要
    • 摘要: 为进一步研究水稻OsTZF3基因的生物学功能，利用日本晴基因组的OsTZF3基因序列，构建了OsTZF3的CRISP/Cas9敲除表达载体pKTZF3和过量表达载体pCXUN-TZF3；并通过农杆菌介导法导入粳稻日本晴胚性愈伤组织中，分别获得了pKTZF3和pCXUN-TZF3转化再生植株55个和42个克隆；进一步通过PCR法鉴定了35个克隆转pKTZF3植株和10个克隆转pCXUN-TZF3植株。

       

      Abstract: In order to further study the biological function of OsTZF3 gene in rice, the CRISP/Cas9 knockout expression vector pKTZF3 and overexpression vector pCXUN-TZF3 of OsTZF3 were constructed by using the sequence of OsTZF3 gene in Nipponbare. 55 transformants of pKTZF3 and 42 clones of pCXUN-TZF3 were obtained through Agrobacterium-mediated transformation. Among them, 35 clones of pKTZF3 and 10 clones of pCXUN-TZF3 were further identified by PCR detection.

       

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