Abstract: The ratooning ability of sterile line Minhong 249S, the suitable stubble height and yield potential for mechanized production of Minhongliangyou 727 as ratooning rice in the first season were compared. The results showed that Minhong 249S was a sterile line with strong ratooning ability. Its ratooning ability was 1.89, which was 13.17% higher than that of Jiafuzhan (1.67), with significant difference. Minhongliangyou 727 was suitable for ratooning rice cultivation with low stubble. When the stubble height was 15 cm, its yield components were coordinated and with high yield in the ratooning season. The yield of Minhongliangyou 727 in the first season, ratooning season and the whole year were 597.87 kg, 320.05 kg and 917.92 kg per 667 m², respectively, which was similar to Luyoumingzhan, the main popularized ratooning rice variety with low stubble that suitable for mechanized production. It was suitable for the application and popularization for mechanized production of ratooning rice with low stubble.