Abstract: In order to effectively preserve the germplasm resources of Anoectochilus formosanus, the method of combining the isolated bagging planting with special formulated nutrient solution developed by irrigation was adopted. The techniques of bagging planting and preservation for germplasm resources of Anoectochilus formosanus were summarized from the aspects of the collection of germplasm resources, the selection of substrate and implements for planting and preserving, bagging planting method and the preservation and management of germplasm. The germplasm resources preserved by the techniques of bagging planting and preservation for germplasm resources of Anoectochilus formosanus would not be easy to be damaged by plant diseases and insect pests, and the process of germplasm planting and preservation had no need of additional watering and topdressing, as well as the management was simple, which had the advantages of simple operation, low preservation cost and high survival rate.