Abstract: The optimal processing conditions of abalone prepared from vacuum tank were studied in this paper. Single factor experiment was used to study the effects of steaming time, vacuum preparation time, vacuum preparation temperature and vacuum degree on abalone quality. Based on the single factor experiment, with the content of amino acid nitrogen in the abalone prepared from tank as the response value, the preparation temperature, preparation time and vacuum degree were selected to conduct the three-factor and three-level response surface analysis test, so as to optimize the best processing technology of the abalone prepared from tank and carry out the verification test. The results showed that the main order of factors affecting the content of amino acid nitrogen in the abalone prepared from tank was the preparation temperature > preparation time > vacuum degree. The best processing conditions for refining abalone were the steaming time of 8 min, the preparation temperature of 30℃, the preparation time of 7.3 h, and the vacuum degree of 0.069 MPa. Under this condition, the the content of amino acid nitrogen of the abalone prepared from vacuum tank was 58.60 mg·hg^-1, and the predicted value was 58.56 mg·hg^-1,showing that the measured value was basically consistent with the predicted value.