Abstract: By taking Kyoho grape as the material, the grapevines were sprayed with self-made agricultural enzyme, thus to study the effects of self-made agricultural enzymes on the sugar content of grape fruits and the activities of enzymes related to sugar metabolism. The results showed that the total sugar content of grape fruits treated with enzymes was higher than that sprayed water control (CK) at 45~105 days after anthesis.The treatment of 400 times concentration of B enzyme (B2) and 300 times concentration of A enzyme (A1)had better effect,Their total sugar content of grape fruit were 23.09% and 20.09% higher than sprayed water control (CK) respectively at 105 days after anthesis. Enzyme treatment had a significant effect on the activity of enzymes related to glucose metabolism, especially in the early stage of fruit development (45-75 d after flowering). The enzyme activities of acid invertase (AI), neutral invertase (NI) and sucrose synthase (SS) in each treatment were significantly higher than those in the spraying water control, but with the ripening of grape fruits, there was no significant difference between the activities of various enzymes related to glucose metabolism (CK) and the treatment of each enzyme after 90 days after flowering.