Abstract: In order to select the best medium for the spheroid differentiation culture of Platycerium bifurcatum, a technical system for the spheroid differentiation culture of Platycerium wallichii Hook. was established. The green globularbodies (GGB) germinated from Platycerium bifurcatum spores were used as the culture material for bud differentiation, and the effects of basic medium, IBA, activated carbon and white sugar on the spheroid differentiation culture of Platycerium wallichii Hook. were studied by using the orthogonal design method. The results showed that the primary and secondary relationship of the effect of each factor on the spheroid differentiation culture of Platycerium wallichii Hook. was basic medium > IBA > AC > white sugar. The medium formula suitable for the GGB differentiation of Platycerium bifurcatum was selected as HD₂ basic medium+ IBA 0.5 mg·L^-1+AC 0.5 mg·L^-1+white sugar 30.0 g·L^-1. The culture was conducted for 56 d and the coefficient of bud differentiation was up to 6.7, which effectively improved its reproductive efficiency and provided technical guarantee for large-scale culture of seedlings.