Abstract: Genetic diversity of 35 major cultivars of Gerbera jamesonii Bolus in Fujian was analyzed by ISSR technology. 15 random primers with good polymorphism were selected from 100 ISSR primers, and were amplified on the genomic DNA of 35 Gerbera jamesonii Bolus. A total of 78 clear and distinguishable bands were obtained, of which 70 were of polymorphism, and the percentage of polymorphic sites reached 89.7%. The DPS software was used for the statistical analysis, and the results showed that the genetic similarity coefficients among the varieties ranged from 0.296 8 to 0.750 0. When the threshold value was 0.420 0, the 35 Gerbera jamesonii Bolus could be divided into four categories. When the genetic similarity coefficient was 0.491 4, the Class I Gerbera jamesonii Bolus could be further divided into four subclasses. The clustering results showed that the color of flower heart and the type of double petals could be used as a basis for the classification of genetic relationship of Gerbera jamesonii Bolus, and the varieties from the same source tended to cluster together, which had a certain corresponding relationship with the clustering results of agronomic traits.