Abstract: In order to screen the best explant for the tissue culture and rapid propagation of Cymbidium floribundum Lindl., and the best medium formula for the seed inducible proliferation and rooting of Cymbidium floribundum Lindl., three kinds of capsule pods of Cymbidium floribundum Lindl. with different maturity were used as explants to study the effects of pods with different maturity on the inducing culture of Cymbidium floribundum Lindl. By using MS as the basal medium and adding carrageenan, the effects of different concentrations of 6-BA, NAA, IBA, activated carbon and banana paste on the induction and proliferation of cluster buds of Cymbidium floribundum Lindl. were studied by orthogonal experiment with four factors and three levels. The effects of different hormone combination of IBA, NAA and activated carbon on the rooting culture of cluster buds of Cymbidium floribundum Lindl. were studied by using 1/2 MS as the basal medium and adding sugar, carrageenan and banana paste. The results showed that the fruit pods of mature and indehiscent capsules of Cymbidium floribundum Lindl. were the best explants for the tissue culture and rapid propagation, with short induction time, high induction rate, low infection rate and good growth. The optimal medium for the induction and proliferation of cluster buds of Cymbidium floribundum Lindl. was MS+6-BA 2.0 mg·L^-1+NAA 0.15 mg·L^-1+banana paste 120 g·L^-1+sucrose 35 g·L^-1+carrageenan 7.0 g·L^-1, and the multiplication coefficient reached 3.1. The optimal medium for the rooting and strong seedling was 1/2 MS basal medium+NAA 0.9 mg·L^-1+IBA 0.9 mg·L^-1+banana paste 120 g·L^-1+sucrose 20 g·L^-1+carrageenan 7.0 g·L^-1. Under this culture condition, the root system of Cymbidium floribundum Lindl. was strong and neat, and the rooting percentage could reach 100%.