Abstract: This study aimed to establish a method for the determination of chloramphenicol in feed by using QuEChERS combined with the high performance liquid chromatography-tandem mass spectrometry method. After water dispersion, the samples were extracted with acetonitrile. And then after the purification by QuEChERS method, the samples were determined by high performance liquid chromatography-tandem mass spectrometry and quantified by external standard method. By using the ultrapure water and methyl alcohol as mobile phases, the gradient elution was carried out by Hypersil GOLD C18 column (1.9 μm, 2.1 mm×100 mm). And then the qualitative and quantitative analysis was carried out by using the electrospray ionization (ESI^-) and anion scanning multiple reaction monitoring (MRM) modes. The results showed that the linear relation of chloramphenicol was good in the range of 5.0-200.0 ng·mL^-1 (r=0.999 2). The recoveries at the adding levels of 10.0, 20.0 and 50.0 μg·kg^-1 ranged from 84.2% to 89.7%, the relative standard deviations (n=6) were 6.5%-8.8%, the limits of detection (based on S/N>3) were 0.2 μg·kg^-1, and the limits of quantitation (based on S/N>10) were 0.6 μg·kg^-1. The method had good precision, high sensitivity and could be used to determine the chloramphenicol in feed simply and accurately.