Abstract: Aeromonas veronii JY01 is a strain of dominant gram-negative bacterium isolated from the lesions of goldfish, such as rotten tails, rot, and ulcers in a farm in Fuzhou. In this study, the whole genome of the strain of Aeromonas veronii JY01 was sequenced based on the IlluminaPE150 sequencing platform and Pacbio sequencing platform. The sequencing data was assembled and analyzed, the gene prediction and functional annotation were performed by bioinformatics methods, meanwhile the prediction of pathogenic genes and drug resistance genes were carried out. The results showed that the genomic sequence length of the strain of Aeromonas veronii JY01 was 4.97 Mb, and the GC content was 58.17%. A total of 4 601 encoding genes, 31 rRNA, 16 gene islands and 124 tRNA were predicted, among which, 3 461 genes were annotated in COG, 3 082 genes were aggregated in GO cluster analysis, and 4 345 genes were enriched in KEGG metabolic pathway. It was predicted that the strain of Aeromonas veronii JY01 carried 13 virulence factors including 240 related virulence genes, and 9 types of antibiotics resistance including 175 antibiotic resistance genes.