Abstract: In order to establish the rapid propagation technology system of Sedeveria pink ruby by high-efficient tissue culture, and provide the technical support for industrialized production, the tissue culture and rapid propagation technology of Sedeveria pink ruby was studied by using the young leaves of Sedeveria pink ruby as the explants. The results showed that 75% alcohol soaked for 60 s and 0.1% mercury bichloride treated for 10 min were the best disinfection treatments for the explants of Sedeveria pink ruby. The optimal culture medium for callus induction of Sedeveria pink ruby was MS+1.5 mg·L^-1 6-BA+0.5 mg·L^-1 NAA (pH=6.0), with the induction rate of 83.3%. The optimum culture medium for bud differentiation from callus was MS+0.6 mg·L^-1 6-BA+0.2 mg·L^-1 NAA (pH=6.0), with the differentiation rate of 77.78%. The optimal culture medium for rooting was 1/2 MS+2.0 g·L^-1 activated carbon (pH=6.0), and the rooting rate could reach 70.00%.