枯草芽孢杆菌SB13产内切葡聚糖酶的促进因子筛选及条件优化

官雪芳; 王琦; 徐庆贤; 孙美玲; 林斌; 黄菊青

doi:10.13651/j.cnki.fjnykj.2021.12.007

枯草芽孢杆菌SB13产内切葡聚糖酶的促进因子筛选及条件优化

Screening of the Promoting Factors and Optimization of the Conditions for the Production of Endoglucanase by Bacillus Subtilis SB13

摘要

摘要: 为提高枯草芽孢杆菌内切葡聚糖酶的分泌水平，对枯草芽孢杆菌SB13产内切葡聚糖酶的促进因子进行了筛选，并通过正交试验进行条件优化，结果表明：MgSO₄、VB₁、VB₂和大豆多糖对菌SB13产内切葡聚糖酶均有显著的促进作用，各因子对菌SB13产内切葡聚糖酶的最佳促进浓度分别为（w/v）：MgSO₄ 0.6%、VB₁ 0.2%、VB₂ 0.4%和大豆多糖 0.8%，正交试验法L₉3⁴表明菌SB13最优促酶因子组合为VB₁ 0.2%、VB₂ 0.5%、Mg²⁺ 1.6%、大豆多糖0.4%，优化获得最优促进温度为30℃、pH值为7.0。通过对促进因子的筛选及条件优化，最终使菌SB13产内切葡聚糖酶活力分别提高了200%，为高内切葡聚糖酶水平的芽孢杆菌制剂生产及应用提供参考依据。

Abstract: In order to improve the secretion level of endoglucanase from Bacillus subtilis, the promoting factors for the production of endoglucanase by Bacillus subtilis SB13 were screened, and the condition optimization was carried out by orthogonal test. The results showed that MgSO₄, VB₁, VB₂ and soybean polysaccharides all had significant promoting effects on the production of endoglucanase by Bacillus subtilis SB13. The optimal promoting concentrations of each factor for the production of endoglucanase by Bacillus subtilis SB13 were (w/v): 0.6% MgSO₄, 0.2% VB₁, 0.4% VB₂, and 0.8% soybean polysaccharides, respectively. The orthogonal test L₉3⁴ showed that the optimal combination of enzyme-promoting factors for Bacillus subtilis SB13 was 0.2% VB₁, 0.5% VB₂, 1.6% Mg²⁺, and 0.4% soybean polysaccharides. The optimal promotion temperature was 30℃ and pH value was 7.0. By screening the promoting factors and optimizing the conditions, the activity of endoglucanase produced by Bacillus subtilis SB13 was increased by 200%, which provided reference for the production and application of Bacillus preparations with high level of endoglucanase.

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