林国钦, 张婷, 左杰, 何锦欣, 蓝灿华, 田宝玉. 产IAA根内生菌的分离鉴定及对小麦促生效果[J]. 福建农业科技, 2022, 53(4): 10-17. DOI: 10.13651/j.cnki.fjnykj.2022.04.002
    引用本文: 林国钦, 张婷, 左杰, 何锦欣, 蓝灿华, 田宝玉. 产IAA根内生菌的分离鉴定及对小麦促生效果[J]. 福建农业科技, 2022, 53(4): 10-17. DOI: 10.13651/j.cnki.fjnykj.2022.04.002
    shu
    LIN Guo-Qin, ZHANG Ting, ZUO Jie, HE Jin-Xin, LAN Can-Hua, TIAN Bao-Yv. Isolation and Identification of IAA-producing Endophytic Bacteria and Its Growth-promoting Effect on the Growth of Wheat[J]. Fujian Agricultural Science and Technology, 2022, 53(4): 10-17. DOI: 10.13651/j.cnki.fjnykj.2022.04.002
    Citation: LIN Guo-Qin, ZHANG Ting, ZUO Jie, HE Jin-Xin, LAN Can-Hua, TIAN Bao-Yv. Isolation and Identification of IAA-producing Endophytic Bacteria and Its Growth-promoting Effect on the Growth of Wheat[J]. Fujian Agricultural Science and Technology, 2022, 53(4): 10-17. DOI: 10.13651/j.cnki.fjnykj.2022.04.002
    shu

    产IAA根内生菌的分离鉴定及对小麦促生效果

    Isolation and Identification of IAA-producing Endophytic Bacteria and Its Growth-promoting Effect on the Growth of Wheat

      摘要
    • 摘要: 生长素在植物生长和发育中扮演着至关重要的作用。吲哚乙酸(IAA)的合成普遍被认为是植物根际细菌和根内生菌促进植物生长最为重要的机制之一。通过对番茄根产IAA内生菌菌株进行分离鉴定并进行促生效果研究,以期为开发生物肥料提供参考依据。采用组织研磨培养法,以TSA培养基为分离培养基,从番茄根分离纯化得到12株番茄根内生菌,并利用16S rDNA序列分析对菌株进行种属鉴定;随机选择3株内生菌菌株mr7、mr31及B21,进一步研究其产IAA能力以及对小麦的促生效果。结果表明:经16S rDNA序列分析鉴定,mr7、mr89为大肠杆菌属Enterobacter,mr31、mr58、B21、GG1、M2、M3、r11、F127、F165、F170为芽孢杆菌属Bacillus;其中mr7、mr31及B21分别为阴沟肠杆菌Enterobacter cloacae、蜡样芽孢杆菌Bacillus cereus和巨大芽孢杆菌Bacillus megaterium。菌株mr7、mr31和B21都具有直接利用色氨酸合成IAA的能力;其中,菌株mr7的IAA产量为67.79 mg·L-1、菌株mr31的IAA产量为86.00 mg·L-1、菌株B21的IAA产量为15.48 mg·L-1。平板试验结果表明,菌株mr7及菌株B21处理后的效果比菌株mr31好,但各菌株处理后小麦的根长及株高均低于对照,菌液不同组分及不同浓度处理后对小麦的促生效果无显著差异;盆栽试验结果表明,3株菌株处理后小麦的叶长、根长及株高均有一定的提高,并且主要促进小麦根的生长,其中以菌株B21的促生效果最佳,同一菌株使用7种不同接种方法处理后各方法间无显著差异。

       

      Abstract: Auxin plays an important role in the growth and development of plant. The synthesis of indole acetic acid (IAA) is generally considered to be one of the most important mechanisms for the rhizosphere bacteria and endophytic bacteria of plant to promote the growth of plant. In order to provide reference for the development of biological fertilizer, the strains of endophytes producing IAA from tomato roots were isolated and identified and their growth-promoting effect was studied. By using the tissue grinding culture technique, twelve strains of tomato endophytic bacteria were isolated and purified from tomato roots with TSA medium as the isolating medium. The genus identification of the strains was carried out by means of 16S rDNA sequence analysis. Three strains of tomato endophytic bacteria including mr7, mr31 and B21 were randomly selected to further study their capacity of producing IAA and the growth-promoting effect on wheat. The results showed that according to 16S rDNA sequence analysis, mr7 and mr89 belonged to Enterobacter, while mr31, mr58, B21, GG1, M2, M3, r11, F127, F165 and F170 belonged to Bacillus, among which mr7, mr31 and B21 were Enterobacter cloacae, Bacillus cereus and Bacillus megaterium, respectively. The strains of mr7, mr31 and B21 had the ability to synthesize IAA directly from threonine, among which the IAA yield of strain mr7, mr31 and B21 was 67.79 mg·L-1, 86.00 mg·L-1 and 15.48 mg·L-1, respectively. The results of plate test showed that the effect of strain mr7 and strain B21 after treatment was better than that of strain mr31, but the root length and plant height of wheat treated with each strain were lower than those of the control group, and there was no significant difference in the growth-promoting effect on wheat treated with different components and concentrations of bacteria solution. The results of pot experiment showed that the leaf length, root length and plant height of wheat treated with the three strains were improved to a certain extent, and they mainly promoted the growth of wheat roots, among which the strain B21 had the best growth-promoting effect. And there was no significant difference when treated with seven different inoculation methods for the same strain.

       

      • HTML全文
      • 参考文献(34)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回