Abstract: UDP glucose dehydrogenase (UDP-GlcDH) can catalyze UDP glucose to UDP glucuronic acid, which is an essential precursor for the formation of polysaccharide. The UDP-GlcDH gene sequence of Aspergillus flavus was used as the template, and the cDNA sequence of UDP-GlcDH gene from Ganoderma lucidum was obtained by searching the EST library of Ganoderma lucidum through the electronic cloning method, and then verified by using the RT-PCR testing. The bioinformatics method was used to analyze the UDP-GlcDH gene, showing that the cDNA sequence was 1 591 bp in length, which contained 471 amino acid sequences with the molecular weight of 51.8 kD and PI of 6.29. The UDP-GlcDH gene was found in the endoplasmic reticulum, which was highly homologous to the amino acid sequence encoded by UDP-GlcDH gene in the fungi such as Dichomitus squalens and Coriolus versicolor.