Abstract: In order to study the seedling propagation and the green control technology for disease and insect pest of Dendrobium officinale Taihu No.1, the effects of different medium formula, the frequency of rich water, pesticide formula and substrate ratios on the growth index and pest control effect of Taihu No.1 were studied. The results showed that: the No.9 medium (1/2 MS+6-BA 3 mg·L^-1+NAA 1 mg·L^-1+banana slurry 100 g·L^-1L) was the most favorable for the growth of tissue culture seedling of Taihu No.1. During the cultivation process, the seedlings of Taihu No.1 had the best plant height and stem diameter at the frequency of rich water every 5 days. Spraying 1 000 times liquid of organic polypeptidase No.1 could effectively control the occurrence of seedling sclerotium disease, soft rot disease and snail damage. And he substrate ratio of pine bark to fir bark (being 1∶1) had the best effect on the seedling growth.