Abstract: Antrodia cinnamomea was a unique fungus in Taiwan of China, with extremely high medicinal and commercial value. The solid-state culture of petri dish has become one of the main artificial culture methods for Antrodia cinnamomea. And screening the optimal culture medium was the key to the culture method. Firstly, the strain BX1119.1 was isolated from the fruiting body of Antrodia cinnamomea cultured from the camphor wood, which was identified as Antrodia camphorata through the morphological characteristics, ITS identification and phylogenetic analysis. Then, with the wheat bran as the basic component, five kinds of grains were added as the carbon sources at the ratio of 1∶1 (w/w) to obtain the grain matrix by mixing. After the inoculation with the identified strains, the strains were cultured for 20 days by using the grain petri-dish culture method, and the mycelial growth diameters were measured on the 5 th, 8 th, 11 th, 14 th, 17 th and 20 th days after the inoculation. The content of triterpenoids in Antrodia camphorata cultured in different grain substrates was determined by using the perchloric acid-vanillin chromogenic method. The results showed that the optimum carbon source for the growth of Antrodia camphorata was yellow rice powder, and the optimum carbon source for the biosynthesis of triterpene was the naked oats flour. The results of this study provided a new solution for the selection of solid matrix in the artificial culture of Antrodia camphorata.