Abstract: Due to the usage of related drugs such as antibiotics in fisheries, the aquatic bacteria such as Vibrio vulnificus have developed the drug resistance. Enhancing the bactericidal effect of the existing antibiotics was an important way to solve this problem. The Vibrio vulnificus at the plateau phase was treated with various aminoglycoside antibiotics under the low ion and physiological osmotic pressure environment, and then the intake of aminoglycoside antibiotics by Vibrio vulnificus under the low ion and physiological osmotic pressure environment was determined. The results showed that the low ion shock could effectively enhance the killing efficacy of aminoglycoside antibiotics against Vibrio vulnificus, and the bactericidal promoting effect had an antibiotic concentration and time dependent manner. The molecular mechanism of low ion treatment which promoted the aminoglycoside antibiotics to kill the Vibrio vulnificus was to increase the uptake of antibiotics by the bacteria. This study laid a foundation for further revealing the mechanism of bacterial resistance and provided a feasible way to solve the problem of bacterial resistance.