Abstract: In order to obtain the lactic acid bacteria strains with excellent acid and bile salt tolerance, the lactic acid bacteria were isolated from the commercial fermented vegetable samples and preliminarily identified by using the morphological observation and sugar fermentation test. Then, the screening of acid and bile salt resistance and the re-screening test of artificial gastrointestinal juice were carried out. The results showed that 11 of the 18 strains of lactic acid bacteria had high acid and bile salt tolerance. After the re-screening of artificial gastrointestinal juice, the survival rate of 4 strains was higher than 30%, and the survival rate of the strain cf12 in artificial gastric juice reached 95%. The survival rates of the strains treated with the artificial intestinal juice for 4 h and 8 h were 173% and 194%, respectively. The concentration of bacterial suspension did not decrease but increased, indicating that the strain had strong tolerance to the low acidic environment and high bile salt environment. The 16S rDNA molecular biological identification of the four strains of lactic acid bacteria showed that the strain cf10 was Lactobacillus plantarum, the strains cf9 and cf12 were Lactobacillus brevis, and the strain cf18 was Lactobacillus parabrevis.